保护地蔬菜栽培不同灌水方法对表层土壤盐分含量的影响

通过 3年连续保护地栽培蔬菜小区试验 ,对滴灌、渗灌、沟灌 3种灌水方法的土壤盐分积累状况进行了比较研究。试验后 0～ 2 0 cm土层土壤全盐含量以沟灌最高 ,渗灌次之 ,滴灌最低 ;滴灌土壤 p H下降幅度明显低于渗灌和沟灌土壤。在 0～ 2 0 cm土层内全盐含量呈幂指数形式分布 ,即地表处含量最高 ,随深度增加逐渐下降 ;而土壤 p H则随深度增加而直线上升。另外 ,土壤中可溶性盐的阴离以 NO-3 为主 ,阳离子以 Ca2 + 为主。这说明选择合理灌水方法 ,是防止土壤退化、提高保护地作物产量和质量的有效途径。     

蔬菜种子物理特性和空气动力学特性试验研究

蔬菜穴盘精量播种是蔬菜工厂化育苗的关键问题,而气力式播种是广泛采用的精量播种方法之一.为此,通过实验研究了黄瓜、蕃茄、茄子和辣椒等蔬菜种子的物理性质和空气动力学特性,测定了形状、尺寸、千粒质量、孔隙率、休止角、漂浮速度和漂浮系数等有头稀理特性,作为设计蔬菜穴盘气力式精量播种装置结构和性能参数的依据.     

基于距离反比法的土壤盐分三维空间插值研究

以新疆兵团一块面积约70 hm2的盐碱地为研究对象,采用EM38与土钻取样相结合的方法得到了126个点不同土层(0~200 cm)的1 386个土壤盐分数据,应用三维距离反比法(3D-IDW)对土壤盐分进行了空间插值,探讨了垂向坐标扩大倍数和搜索点数对插值结果精度的影响。结果表明,研究区0~140 cm土层盐分平均含量较高,为1.84~2.11 g/kg,盐分变异较大,而140~200 cm土层盐分平均含量较低,为1.74~1.79 g/kg。所有土层盐分含量的统计特征值(平均值、标准差和变异系数等)均随土层加深而呈现递减的趋势。土壤盐分实测值和估计值的均方根误差随垂向坐标扩大倍数的增大而减小,随搜索点数的增加而增大,其值在0.1~0.4 g/kg范围内变化,当垂向坐标扩大300倍、搜索点数为6个时,插值效果较优。采用确定的参数对研究区的土壤盐分进行了三维空间插值,结果表明土壤盐分空间分布特征与实测值比较吻合,大部分区域土壤盐分含量小于2.5 g/kg,靠近北部和南部边界区土壤含盐量较低,属于非盐化土区,而大于4 g/kg盐化土主要分布在中间和南部局部区域。研究区80%土壤为非盐化土和轻度盐化土,20%为中度和重度盐化土。影响该区盐化土分布的主要因素有灌溉、局部地形、粘土层位置、地下水埋深和矿化度。当不同方向的取样间距相差很大时,选取合适的垂向坐标扩大倍数和搜索点数对保障3D-IDW法的插值结果精度至关重要。     

MicroRNA-9-5p mediates sympathetic overactivity by targeting KCNN3 in paraventricular nucleus of rats with T2D

AIM To investigate whether microRNA-9-5p （miR-9-5p） mediates sympathetic overactivity by targeting KCNN3 （potassium intermediate/small conductance calcium-activated channel, subfamily N, member 3） gene,which encoded small-conductance calcium-activated potassium channel 3 （SK3） protein, in paraventricular nucleus （PVN） of rats with type 2 diabetes mellitus （T2D）. METHODS A rat model of T2D was established by high-fat diet combined with intraperitoneal injection of 30 mg/kg streptozotocin. The levels of miR-9-5p and KCNN3 mRNA in PVN were detected by real-time PCR. The relationship between KCNN3 and miR-9-5p was predicted by TargetScan. Recombinant adeno-associated virus （rAAV）-miR-9-5p or KCNN3 were bilaterally microinjected into the PVN to observe the changes in plasma glucose levels and sympathetic drive indicators. The number of FosB and SK3 positive cells was measured by immunofluorescence staining. The protein expression of SK3 was determined by Western blot. The relationship between KCNN3 and miR-9-5p were confirmed by cell transfection and dual-luciferase reporter assay. RESULTS Compared with the rats in diabetes control （DC） group, the blood glucose, sympathetic drive indexes and the level of miR-9-5p in PVN were significantly increased, while the SK3 expression in PVN was obviously reduced in the diabetes mellitus （DM） rats. After microinjecion of rAAV-miR-9-5p in PVN, the sympathetic drive indexes, blood glucose, and the number of FosB-positive cells were increased significantly, but the SK3 protein expression was significantly reduced （P<0.05）. However, up-regulation of KCNN3 in PVN had the opposite effect. These responses were obviously enhanced in DM rats compared with DC rats. The results of cell transfection and dual-luciferase reporter assay demonstrated that miR-9-5p bound to the 3’-UTR of KCNN3 and inhibit its expression. CONCLUSION miR-9-5p was up-regulated in PVN of the rats with T2D, and it may mediate sympathoexcitation by targeting KCNN3.     

基于三维点云的蔬菜大棚杂草识别方法

为实现蔬菜大棚内自动化除草的目的,针对其中的杂草识别环节,提出了一种基于三维点云的新型蔬菜大棚杂草识别方法。采用RGB-D相机获取青菜田、生菜田的三维点云图像,采用超绿色算法去除其中的土壤等背景,采用体素滤波法在保留点云图像形状特征的同时降低点云数量,然后采用欧式聚类法分割出单株青菜和单棵杂草的点云簇,分别计算得到每个点云簇的最高点的Z坐标值,最后结合深度信息Z坐标值实现蔬菜大棚杂草识别。结果表明:这种基于三维点云的杂草识别方法能够有效的识别出杂草,识别率为86.48%。该方法能够对蔬菜大棚中的杂草进行准确识别,为蔬菜大棚自动化除草提供有效的解决方案。     

十字花科蔬菜害虫种类及综合治理技术

针对近年来江门市蔬菜种植过程中害虫种类繁多、化学农药使用量增加,且抗药性不断增强,防治工作困难的现状,进行了多年实践,在分析江门市十字花科蔬菜害虫发生种类及发生特点的基础上,提出通过采用清除残株落叶、合理轮作等农业防治措施,设置杀虫灯、黄板、性诱剂等物理防治措施,释放寄生蜂、捕食性天敌等生物防治措施,辅之以合理使用高效、低毒、低残留化学农药的综合治理技术,实现了害虫防控总体防效达到90%、化学农药使用量减少30%以上、产量增加5%以上的良好效果,值得大面积推广。     

不同植物油对大规格吉富罗非鱼生长性能、组织脂肪酸组成的影响

本试验旨在探究大规格吉富罗非鱼(GIFT)对不同植物油的利用效果,筛选出大规格吉富罗非鱼饲料中合适的植物脂肪源。分别以大豆油(SO)、棕榈油(PO)、棉籽油(CO)、菜籽油(RO)、磷脂油(SL)和大豆油∶棕榈油∶棉籽油∶菜籽油∶磷脂油=1∶1∶1∶1∶1的混合油(MIX)为脂肪源配制6种等氮等脂的试验饲料,饲喂初始体重为(229.92±0.31)g的吉富罗非鱼8周。每种试验饲料投喂4个网箱(重复),每个网箱放养50尾。结果表明:对于增重率和特定生长率,棉籽油组和混合油组显著低于菜籽油组(P0.05)。菜籽油组饲料系数显著低于混合油组(P0.05),其他各组间无显著差异(P0.05)。混合油组蛋白质效率显著低于菜籽油组和磷脂油组(P0.05)。混合油组肝体指数显著高于棕榈油组和菜籽油组(P0.05)。大豆油组、菜籽油组、棕榈油组和磷脂油组在增重率、特定生长率和饲料系数方面没有显著差异(P0.05)。大豆油组和菜籽油组肌肉饱和脂肪酸总量显著低于其他各组(P0.05)。对于肌肉中n-3系脂肪酸/n-6系脂肪酸,菜籽油组显著高于其他各组(P0.05),棉籽油显著低于其他各组(P0.05)。棉籽油组肝脏饱和脂肪酸总量显著高于其他各组(P0.05),菜籽油组则显著低于其他各组(P0.05);对于肝脏n-3/n-6,菜籽油组显著高于其他各组(P0.05)。各组肌肉脂肪酸组成与饲料脂肪酸组成的相关系数均高于肝脏脂肪酸组成与饲料脂肪酸组成的相关系数。在饲料脂肪酸组成与肌肉或肝脏脂肪酸组成的相关系数中,均以棕榈油组最大,磷脂油组最小。由此得出,大豆油、棕榈油、菜籽油和磷脂油可以作为大规格吉富罗非鱼(体重为230~790 g)饲料中良好的脂肪源。大规格吉富罗非鱼肌肉中脂肪酸组成一定程度上被饲料脂肪酸组成影响,但肝脏脂肪酸组成与饲料脂肪酸组成的相关性较小。     

蔬香糟汁的工艺研究

蔬香糟汁是在糟汁的基础上加入黄酒、蔬菜汁等原料经过调配而成的,其成品色泽深褐,香味浓郁,口味独特,适宜于大众口味。从蔬香糟汁的配方设计、各原料的比例、加工工艺流程和操作中需要特别注意的问题等诸多因素进行详细分析;重点研究了香糟卤的用量、黄酒的用量和蔬菜汁的用量对成品口味的影响,通过正交试验确定了最佳搭配比例。     

基于RFID的无公害蔬菜质量安全管理系统设计与实现

以RFID技术为依托,以农超对接中的“基地-加工企业-超市”为主要模式,围绕无公害蔬菜生产、包装、销售的主要环节,建立基于RFID的无公害蔬菜质量安全管理系统,实现对无公害蔬菜从产地到加工经营企业整个流程实施有效监管和追溯,增加对无公害蔬菜产品农药残留检测的密度和范围,及时依据检测结果做出准否上市的正确判断.     

Mechanism of quercetin improving rat coronary artery myogenic response under high glucose

AIM: To investigate the mechanism of quercetin improving rat coronary artery myogenic response under high glucose (HG) by measuring muscle tension of coronary arterial ring and recording voltage-gated K⁺ channel (Kv) current of coronary artery smooth muscle cells by whole cell patch clamp. METHODS: The coronary rings from the normal SD rats were acutely isolated, and then divided into 6 groups: (1) control group; (2) HG group; (3) HG+low dose (3 μmol/L) of quercetin group; (4) HG+moderate dose (10 μmol/L) of quercetin group; (5) HG+high dose (30 μmol/L) of quercetin group; (6) HG+C6303 (PKC inhibitor)+high dose of quercetin group. Determinations of coronary artery response to vasoconstrictor (60 mmol/L KCl or 0.1 mmol/L U46619) or vasodilator (ACh at 10^-9~10^-5 mol/L) were performed, and the percentage of coronary ring tension was calculated using the contraction as 100% caused by 60 mmol/L KCl. The rat coronary artery smooth muscle cells were acutely isolated for recording the Kv current using whole cell patch clamp. RESULTS: Compared with control group, the contraction amplitudes to 60 mmol/L KCl or 0.1 mmol/L U46619 were significantly increased under HG incubation. Quercetin intervention concentration-dependently reduced the coronary artery contraction amplitude. Incubation of PKC specific inhibitor C6303 attenuated the effect of quercetin. Compared with control group, the diastolic amplitude to ACh decreased significantly in HG group, and quercetin intervention concentration-dependently increased the coronary artery diastolic amplitude. Incubation of PKC specific inhibitor C6303 attenuated the effect of quercetin. Compared with control group, HG incubation inhibited Kv current of coronary artery vascular smooth muscle cells significantly, and quercetin intervention attenuated the inhibitory effect of HG on Kv current intensity. Incubation of PKC specific inhibitor C6303 attenuated the effect of quercetin. CONCLUSION: Quercetin has a protective effect on myogenic response of coronary artery under HG and the effects is related to the increase in Kv current and the activation of PKC in vascular smooth muscle cells.